Cultivation of Chlorella zofingiensis and nutrients removal in dairy wastewater were investigated in bench-scale outdoor ponds in winter, South China. The impacts of the two types of pH regulations, 5 ≈ 6% CO(2) and acetic acid (HAc) on this process were studied. After 6 days cultivation, the removal rates of total nitrogen (TN) and orthophosphate (PO(4)(3-)) using CO(2) regulation were better than those using HAc. The removal rates of PO(4)(3-) and TN were 97.5% and 51.7%, respectively using CO(2) regulation; 79.6% (TN) and 42.0% (PO(4)(3-)) were obtained using HAc regulation. Higher biomass, protein, sugar content, and stable pH control were found using CO(2) regulation. However, significantly higher lipid content (31.8%) was observed using HAc regulation. The dominant differences of fatty acids were the content of C18:1 and C18:3. The growth characteristics and environmental conditions especially during the typical logarithmic phase were also analyzed.
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